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Related Experiment Videos

The nucleolus in telophase, interphase and prophase

M Ashraf, M B Godward

    Journal of Cell Science
    |February 1, 1980
    PubMed
    Summary

    This study reveals that the pale fibrillar material within the nucleolus of Spirogyra is actually decondensed chromatin from the nucleolar-organizing chromosome, crucial for gene transcription. This finding clarifies nucleolar structure and function during the cell cycle.

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    Area of Science:

    • Cell Biology
    • Cytogenetics
    • Algal Biology

    Background:

    • The structure and function of the nucleolus, particularly its relationship with chromosomes, remain areas of active research.
    • Understanding the nucleolar-organizing region (NOR) and its role in ribosome biogenesis is key to cell cycle regulation.

    Purpose of the Study:

    • To investigate the ultrastructural transformations from telophase to interphase in Spirogyra submargaritata.
    • To elucidate the nature of the 'pale fibrillar material' within the nucleolus and its connection to the chromosome.

    Main Methods:

    • Transmission electron microscopy was used to observe cellular ultrastructure.
    • Detailed analysis of chromatid decondensation and nucleolus regeneration from telophase through interphase.

    Main Results:

    • Demonstrated continuity between decondensing telophase chromatids and the pale fibrillar material in the interphase nucleolus.
    • Identified the pale fibrillar material as expanded chromatin of the nucleolar-organizing chromosome, active in transcription.
    • Observed condensed chromosome segments traversing the nucleolonema in early prophase.

    Conclusions:

    • The pale fibrillar material represents decondensed, transcriptionally active chromatin of the nucleolar-organizing region.
    • Proposed a model for nucleolus structure and function based on electron microscopy and current knowledge.
    • Addressed ongoing debates regarding the term 'nucleolar organizer'.

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