Determination of plasma mepindolol levels by high-performance liquid chromatography and electrochemical detection

A method for determining the plasma concentration of the beta-receptor blocking agent mepindolol by high-performance liquid column chromatography (paired-ion chromatography) and electrochemical detection is described. Pindolol is used as an internal standard and the detection limit after extraction of 1 ml of plasma is less than 1 ng of mepindolol. Reproducible results can be obtained with relative standard deviations from replicate analyses of 5 ng/ml plasma samples within +/- 4.7%. The method is also suitable for quantification of plasma pindolol levels with mepindolol as an internal standard.