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Growth characteristics of influenza virus type C in avian hosts. Brief report
Archives of Virology
|January 1, 1978
Abstract:
Influenza virus type C could be propagated to high yield in primary chick embryo kidney cell culture (PCEK) provided that trypsin (2 microgram/ml) was used as a medium supplement. The virus could also be titrated by plaque assay using PCEK host cells and influenza C virus that had been plaque-purified in PCEK cells could then be serially passaged to high titer using the allantoic route of 10--11-day-old embryonated eggs.
Insights
Influenza C virus can be grown to high yields in primary chick embryo kidney cell cultures with trypsin. This method enables plaque assays and high-titer viral propagation in embryonated eggs.
Area of Science:
- Virology
- Cell Culture Techniques
Background:
- Influenza C virus presents unique challenges for propagation and study.
- Efficient methods are needed for high-titer influenza C virus production.
Purpose of the Study:
- To establish a method for high-yield propagation of influenza C virus.
- To enable titration and serial passaging of influenza C virus.
Main Methods:
- Primary chick embryo kidney cell (PCEK) cultures were used for influenza C virus propagation.
- Trypsin supplementation (2 µg/ml) was essential for high-yield virus growth.
- Plaque assays were performed using PCEK cells for virus titration.
- Plaque-purified virus was serially passaged via the allantoic route in embryonated eggs.
Main Results:
- Influenza C virus was successfully propagated to high yield in PCEK cell cultures.
- Trypsin was identified as a critical supplement for virus replication.
- Plaque assays provided a reliable method for virus titration.
- Serial passaging in embryonated eggs resulted in high-titer virus stocks.
Conclusions:
- Primary chick embryo kidney cell culture with trypsin is an effective method for influenza C virus propagation.
- This technique facilitates both titration and large-scale production of influenza C virus for research.