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Methods to quantify silver in autoradiographs

D L Fry, A J Tousimis, T L Talbot

The American Journal of Physiology

|March 1, 1980

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Electron probe X-ray microanalysis (EPA) accurately quantified silver in arterial tissue autoradiographs, outperforming grain counting. EPA offers a reproducible and sensitive method for analyzing radiolabeled tissue samples.

Area of Science:

Biomedical Imaging
Materials Science
Radiochemistry

Background:

Quantifying silver in photographic films and autoradiographs is crucial for analyzing radiolabeled arterial tissue.
Traditional methods like grain counting (GC) and microdensitometry (OD) have limitations in accuracy and reproducibility.

Purpose of the Study:

To evaluate and compare the efficacy of electron probe X-ray microanalysis (EPA) against conventional methods for silver quantification.
To determine the reliability and influencing factors of the EPA technique in this application.

Main Methods:

Developed silver in photographic films (PF) and autoradiographs (AR) of radiolabeled arterial tissue was quantified using three methods: direct grain counting (GC), microdensitometry (OD), and electron probe X-ray microanalysis (EPA).
EPA signal reproducibility was tested across multiple specimen traverses.

Related Experiment Videos

Factors affecting EPA signal, including beam current, silver concentration, beam size, current density, energy, and coating thickness, were investigated.

Main Results:

Electron probe X-ray microanalysis (EPA) data showed a strong positive correlation with microdensitometry (OD) data, exhibiting minimal variance.
Grain counting (GC) data correlated with EPA data but displayed significant variance.
The EPA signal demonstrated high reproducibility, direct proportionality to electron beam current and silver concentration, and insensitivity to beam size, current density, energy above 17 keV, and conductive coating thickness.

Conclusions:

Electron probe X-ray microanalysis (EPA) is a superior method for quantifying silver in radiolabeled arterial tissue autoradiographs compared to grain counting.
EPA offers a precise, reproducible, and reliable analytical technique for biomedical imaging applications involving silver quantification.