Effect of butylated hydroxytoluene on Newcastle disease virus

A study was done to examine the effects of butylated hydroxytoluene (BHT) on purified Newcastle disease virus (NDV). Treatment of the virus with 50 microgram of BHT/ml caused 92% inactivation of the virion infectivity. Virion adsorption to chicken-embryonated cells was inhibited 32% and synthesis of intracellular hemagglutinin was inhibited 29%. Electron microscopy of the BHT-treated virions revealed virion envelope damage. Chicken-embryonated cells treated with 25 microgram of BHT/ml before NDV infection demonstrated 65% inhibition of NDV progeny production.