Lidocaine: effect on phagocytosis and purification of monocytes in bovine peripheral blood

Enumeration of bovine monocytes was determined by latex ingestion, using live cell and fixed cell techniques. Approximately 7.33% of the mononuclear leukocytes were found to phagocytize latex particles by live-cell technique and approximately 7.38% of mononuclear leukocytes phagocytized latex particles by fixed-cell technique. Lidocaine (30 mM) significantly inhibited phagocytosis of latex particles; however, the effects of lidocaine can be reversible. Adherent monocytes can be removed with lidocaine treatment and can be enriched to as much as 90% pure monocytes. Removal of lidocaine was associated with a return to normal cell function.