Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro chemosensitivity tests on xenografted human melanomas

A E Bateman, P J Selby, G G Steel

    British Journal of Cancer
    |February 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.

    Leukemia·2015
    Same author

    The prevalence and outcomes of frailty in older cancer patients: a systematic review.

    Annals of oncology : official journal of the European Society for Medical Oncology·2014
    Same author

    miR-210 is a target of hypoxia-inducible factors 1 and 2 in renal cancer, regulates ISCU and correlates with good prognosis.

    British journal of cancer·2013
    Same author

    Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma.

    British journal of cancer·2012
    Same author

    Adenovirus-mediated hypoxia-targeted gene therapy using HSV thymidine kinase and bacterial nitroreductase prodrug-activating genes in vitro and in vivo.

    Cancer gene therapy·2011
    Same author

    Improving the accuracy of pre-operative survival prediction in renal cell carcinoma with C-reactive protein.

    British journal of cancer·2010

    This study validated an in vitro chemosensitivity assay for malignant melanoma. The assay accurately predicted drug sensitivity in vivo, correlating with clinical outcomes and mouse models.

    Area of Science:

    • Oncology
    • Pharmacology
    • Cancer Research

    Background:

    • Malignant melanoma is a significant health concern.
    • Predicting patient response to chemotherapy is crucial for effective treatment.

    Purpose of the Study:

    • To evaluate an in vitro chemosensitivity test for malignant melanoma cells.
    • To compare in vitro drug sensitivity with in vivo efficacy in a mouse model.

    Main Methods:

    • Malignant melanoma cells from 5 patients were cultured as xenografts in immune-suppressed mice.
    • An in vitro assay exposed tumor cells to 8 chemotherapeutic drugs.
    • Cell survival was assessed using a soft agar colony assay to determine dose-response curves.

    Main Results:

    Related Experiment Videos

  • Significant differences in drug sensitivity were observed among the 8 tested agents.
  • In vitro drug sensitivity results aligned with clinical experience.
  • A significant correlation was found between in vivo (mouse) and in vitro sensitivities for Melphalan and MeCCNU.

    • Conclusions:

    • The developed in vitro chemosensitivity assay shows promise for predicting patient response to chemotherapy.
    • This assay can aid in selecting optimal chemotherapeutic agents for individual malignant melanoma patients.
    • The findings support the utility of this assay in preclinical drug sensitivity testing.