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Related Experiment Videos

Decreasing sample volume used in the "Sequential Multichannel Analyzer Computerized" (SMAC)

R Stamper, D M Robertshaw

    Clinical Chemistry
    |May 1, 1980
    PubMed
    Summary

    Modifications to alkaline phosphatase and creatinine cartridges on the SMAC analyzer allow 18 tests from one prediluted sample. This reduces sample volume and prevents clots, improving efficiency and saving costs.

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    Area of Science:

    • Clinical chemistry
    • Biomedical engineering
    • Laboratory automation

    Background:

    • The Sequential Multichannel Analyzer Computerized (SMAC) is a widely used automated clinical chemistry analyzer.
    • Running multiple tests often requires large sample volumes and can be prone to issues like fibrin clots.
    • Specific cartridges, such as those for alkaline phosphatase and creatinine, may present unique challenges in sample handling.

    Purpose of the Study:

    • To describe modifications to alkaline phosphatase and creatinine cartridges for the 18-channel SMAC analyzer.
    • To enable running all 18 tests using a single, prediluted sample.
    • To reduce the required sample volume and mitigate sample-related issues like fibrin clots.

    Main Methods:

    • The study involved modifying existing alkaline phosphatase and creatinine cartridges for the SMAC system.

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  • The modifications were designed to allow for the use of a commonly prediluted sample for all 18 tests.
  • New components were minimized, and the modifications were implemented rapidly.

    • Main Results:

    • The modified cartridges successfully allowed all 18 tests to be performed with a single prediluted sample.
    • A significant decrease in the required sample volume was achieved.
    • The problem of fibrin clots in the stream-splitter on the main sample line was eliminated.
    • Post-modification performance metrics (accuracy, precision, linearity, carryover) were found to be acceptable when compared to premodification data.

    Conclusions:

    • The described modifications to SMAC cartridges are effective in reducing sample volume and preventing clotting issues.
    • These enhancements improve the operational efficiency of the SMAC analyzer.
    • The modifications offer potential financial savings through reduced reagent consumption and fewer repeat analyses.