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Corticosterone catabolism by mouse liver: interactions between genotype and diet
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|March 1, 1980
Summary
Corticosterone reduction varied between mouse strains, influenced by diet. DBA/2J mice showed greater response to NADPH stimulation after a fat-free diet compared to C57BL/10ScSn mice.
Area of Science:
- Biochemistry
- Genetics
- Animal Models
Background:
- Corticosterone metabolism is crucial for physiological regulation.
- Genetic background of mice can influence metabolic enzyme activity.
- Dietary fat impacts metabolic pathways and enzyme responses.
Purpose of the Study:
- To investigate strain-specific differences in corticosterone reduction.
- To determine the effect of dietary fat on enzyme activity related to corticosterone metabolism.
- To compare the response of different mouse strains to NADPH stimulation.
Main Methods:
- Liver homogenates from C57BL/10ScSn and DBA/2J mice were analyzed.
- Corticosterone reduction assays were performed.
- Reactions were assessed with and without an NADPH-generating system.
- Mice were subjected to a 4% fat diet and a fat-free diet.
Main Results:
- Liver homogenates from C57BL/10ScSn mice exhibited greater corticosterone reduction than DBA/2J mice.
- NADPH stimulation equally enhanced reactions in both strains on a 4% fat diet.
- On a fat-free diet, NADPH significantly boosted reactions in DBA/2J mouse homogenates more than in C57BL/10ScSn.
- C57BL/Tb and CBA/FaCam mice showed similar patterns to C57BL/10ScSn and DBA/2J mice, respectively.
Conclusions:
- Mouse strain significantly impacts corticosterone metabolism.
- Dietary fat manipulation alters the responsiveness of metabolic enzymes to NADPH.
- DBA/2J mice demonstrate a heightened reliance on NADPH-dependent pathways under fat-depleted conditions.