Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metal electronic effects on myoglobin conformational stability

G McLendon, P Murphy

    The Journal of Biological Chemistry
    |May 10, 1980
    PubMed
    Summary

    This study quantifies how changing metals affects myoglobin stability. Metal electronic state influences myoglobin

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Neurology Undergraduate Medical Education: A Scoping Review.

    European journal of neurology·2025
    Same author

    Use of radiological information system data in Clinical Portal database to screen for missed abdominal aortic aneurysms.

    Annals of the Royal College of Surgeons of England·2022
    Same author

    CyBorD-DARA in Newly Diagnosed Transplant-Eligible Multiple Myeloma: Results from the 16-BCNI-001/CTRIAL-IE 16-02 Study Show High Rates of MRD Negativity at End of Treatment.

    Clinical lymphoma, myeloma & leukemia·2022
    Same author

    [The alternative model of personality disorders among the French population: Assessment with brief tools].

    L'Encephale·2022
    Same author

    Canadian Surgery Forum 2018: St. John's, NL Sept. 13-15, 2018.

    Canadian journal of surgery. Journal canadien de chirurgie·2022
    Same author

    2021 Canadian Surgery Forum: Virtual, online Sept. 21-24, 2021.

    Canadian journal of surgery. Journal canadien de chirurgie·2022

    Area of Science:

    • Biochemistry
    • Structural Biology
    • Bioinorganic Chemistry

    Background:

    • Myoglobin's conformational stability is crucial for its function.
    • Understanding how metal ions influence protein structure is key.
    • Previous studies lacked quantitative data on metal substitution effects.

    Purpose of the Study:

    • To quantitatively measure the impact of various metal substitutions on myoglobin conformational stability.
    • To investigate the relationship between metal electronic states and protein energy.
    • To establish a basis for predicting myoglobin stability based on metal properties.

    Main Methods:

    • Performed quantitative measurements of myoglobin conformational stability.
    • Examined metallomyoglobins with diverse metal ions (Fe, Cr, Rh, Mn, Zn, Cu, Ru).
    • Utilized reversible denaturation to assess protein energy dependence on metal electronic state.

    Main Results:

    • Metal substitution generally does not alter protein-denaturant interactions.
    • A direct relationship exists between conformational free energy and metal-imidazole bond strength for trivalent metals.
    • Divalent and trivalent metal derivatives show distinct stability profiles, irrespective of metal-imidazole bond strength.

    Conclusions:

    • Reversible denaturation is a valid method to study metal-dependent myoglobin conformational energy.
    • The electronic state of the metal ion significantly influences myoglobin's conformational stability.
    • Distinct differences in stability are observed between divalent and trivalent metallomyoglobins.

    Related Experiment Videos