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An endothelial cell-derived growth factor

C Gajdusek, P DiCorleto, R Ross

The Journal of Cell Biology

|May 1, 1980

Summary

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Plasma-derived serum (PDS) lacks growth factors. Endothelial cell preincubation modifies PDS to support cell growth via a novel heat-labile polypeptide distinct from platelet-derived growth factor.

Area of Science:

Cell biology
Biochemistry
Growth factor research

Background:

Cell-free plasma-derived serum (PDS) is typically unable to support the proliferation of various cell types, including fibroblasts and smooth muscle cells.
This deficiency is attributed to the absence of essential platelet-derived growth factor (PDGF).

Purpose of the Study:

To investigate the mechanism by which endothelial cells modify PDS to support cell growth.
To characterize the nature of the growth-promoting activity generated by endothelial cells.

Main Methods:

Preincubation of PDS-containing medium with endothelial cells.
Assessing the growth-supporting capacity of the modified medium on 3T3 cells, fibroblasts, and smooth muscle cells.
Characterization of the active polypeptide, including molecular weight estimation, heat stability, and CM Sephadex adsorption properties.

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Main Results:

Endothelial cell preincubation renders PDS capable of supporting the growth of 3T3 cells, fibroblasts, and smooth muscle cells.
The growth-promoting activity is associated with a polypeptide of 10,000–30,000 daltons.
This polypeptide exhibits heat instability and does not adsorb to CM Sephadex, differentiating it from PDGF.

Conclusions:

Endothelial cells secrete a novel growth-promoting polypeptide that modifies plasma-derived serum.
This endothelial cell-derived factor differs significantly from platelet-derived growth factor in its properties.
The findings suggest a distinct pathway for endothelial cell-mediated growth factor production and signaling.