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Bone solubilization by mononuclear cells

W McArthur, A M Yaari, I M Shapiro

Laboratory Investigation; a Journal of Technical Methods and Pathology

|April 1, 1980

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Viable macrophages demonstrate osteolytic activity, releasing calcium from bone in vitro. This bone-dissolving capability is enhanced by endotoxin and is crucial for understanding bone resorption processes.

Area of Science:

Immunology
Cell Biology
Bone Biology

Background:

Mononuclear cells can influence bone metabolism.
The specific role of macrophages in bone resorption requires further elucidation.

Purpose of the Study:

To investigate the osteolytic potential of macrophages.
To identify factors modulating macrophage-mediated bone dissolution.

Main Methods:

In vitro culture of chicken and mouse macrophages with devitalized bone.
Assessment of calcium and 14C-proline release.
Analysis of macrophage surface markers and histochemistry.
Treatment with inactivating agents, endotoxin, parathyroid hormone, and calcitonin.

Main Results:

Related Experiment Videos

Adherent mononuclear cells, identified as macrophages, released calcium and 14C-proline from bone.
Non-macrophage mononuclear cells did not exhibit osteolytic activity.
Macrophage viability and culture duration correlated with bone solubilization.
Escherichia coli lipopolysaccharide significantly increased bone calcium release, while parathyroid hormone and calcitonin had no effect.

Conclusions:

Viable macrophages possess significant in vitro osteolytic activity.
Macrophage-mediated bone dissolution is modulated by inflammatory mediators like endotoxin.
These findings highlight the role of macrophages in bone resorption and its regulation.