Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aldrin epoxidase activity in developing rabbit lung

H M Mehendale

    Pediatric Research
    |April 1, 1980
    PubMed
    Summary

    Pulmonary aldrin epoxidase activity in rabbits increases with age up to 12 weeks, as shown by perfusion studies. In vitro methods did not reflect this age-related increase, suggesting accessibility and cofactor limitations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of cell division in hepatoma cell cultures by chlordecone and carbon tetrachloride combination.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Biochemical studies on the age-related toxicity of galactosamine in primary rat hepatocyte cultures.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Mechanisms and outcomes of drug- and toxicant-induced liver toxicity in diabetes.

    Critical reviews in toxicology·2007
    Same author

    Dose-dependent liver regeneration in chloroform, trichloroethylene and allyl alcohol ternary mixture hepatotoxicity in rats.

    Archives of toxicology·2005
    Same author

    Cytochrome P4502E1 induction increases thioacetamide liver injury in diet-restricted rats.

    Drug metabolism and disposition: the biological fate of chemicals·2001
    Same author

    Diallyl sulfide inhibition of CYP2E1 does not rescue diabetic rats from thioacetamide-induced mortality.

    Toxicology and applied pharmacology·2001

    Area of Science:

    • Environmental Toxicology
    • Pharmacokinetics
    • Mammalian Physiology

    Background:

    • Aldrin epoxidase is a key enzyme in xenobiotic metabolism.
    • Understanding age-related changes in enzyme activity is crucial for risk assessment.
    • Rabbit lungs are a model for studying pulmonary xenobiotic metabolism.

    Purpose of the Study:

    • To investigate the age-dependent changes in pulmonary aldrin epoxidase activity in male rabbits.
    • To compare aldrin epoxidase activity using isolated perfused lung preparations versus in vitro incubation.
    • To identify factors contributing to discrepancies between the two experimental methods.

    Main Methods:

    • Pulmonary aldrin epoxidase activity was measured in male rabbits aged 2-16 weeks.
    • Isolated perfused lung preparations were used to assess in vivo-like activity.
    • In vitro incubation techniques were employed with similar substrate-to-tissue ratios.
    • Lung weight and aldrin epoxidation rates were correlated with age.

    Main Results:

    • Aldrin epoxidase activity in rabbit lungs significantly increased with age, peaking at 12 weeks.
    • Increased lung weight with age did not correlate with enhanced epoxidation.
    • In vitro experiments failed to demonstrate the age-related increase observed in perfusion studies.
    • Extrapolated in vitro epoxidation values were considerably higher than those from perfusion.

    Conclusions:

    • Pulmonary aldrin epoxidase activity exhibits significant age-dependent modulation in male rabbits.
    • Discrepancies between perfusion and in vitro methods suggest limitations in substrate accessibility or cofactor availability in in vitro systems.
    • Further research is needed to elucidate the precise mechanisms underlying these observed differences.

    Related Experiment Videos