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[Glucose determination in the hemolysate, manual analysis]
Schweizerische Medizinische Wochenschrift
|March 29, 1980
Summary
This study presents a manual method for determining glucose in hemolysate using either hexokinase/G6P-DH or glucose dehydrogenase reagents. Both methods show satisfactory correlation with approved and automated glucose assays.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Analytical Chemistry
Background:
- Accurate glucose measurement in hemolysate is crucial for various diagnostic and research applications.
- Manual methods offer an alternative to automated systems, particularly in settings with limited resources.
Purpose of the Study:
- To describe and evaluate a manual method for glucose determination in hemolysate.
- To assess the performance of two enzymatic reagent systems: hexokinase/glucose-6-phosphate dehydrogenase (G6P-DH) and glucose dehydrogenase (Gluc-DH).
Main Methods:
- Manual assay of glucose in hemolysate samples.
- Utilized hexokinase/G6P-DH (e.g., Glucoquant) and glucose dehydrogenase as enzymatic reagents.
- Correlated results with an established approved method and an automated analytical method.
Main Results:
- Both manual methods demonstrated satisfactory correlation with the approved reference method.
- The glucose dehydrogenase method showed a high correlation coefficient (r = 0.98323) with the automated method.
- The hexokinase/G6P-DH method also showed good correlation (r = 0.94172) with the automated method.
Conclusions:
- The described manual method is suitable for glucose determination in hemolysate.
- Both enzymatic reagent systems provide reliable and accurate glucose measurements.
- The manual assay serves as a viable alternative for glucose quantification in hemolysate.