A quantitative assessment of the effect of medium composition on mouse myogenesis in vitro

The effect of variations in medium horse serum (HS) and chick embryo extract on the extent of myogenesis in primary mouse muscle cultures was investigated. Seeding efficiency at 24 h in culture did not vary with medium composition, but total cell number at 8 days was diminished at low embryo extract concentrations. Five media from the range of 16 tested, supported significantly greater myogenesis. Within this group of 5, there was no difference in ability to support myogenesis. The effect of medium composition on myogenesis is discussed.