A centrifugal column assay for thymidylate synthetase using the active site titrant 5-fluoro-2'-deoxyuridylate

Severe folate restriction results in depletion of and alteration in the composition of the intracellular folate pool, moderate sensitization to methotrexate and trimetrexate, upregulation of endogenous DHFR activity, and overexpression of metallothionein II and folate receptor alpha that, upon folate repletion, confer drug resistance to CHL cells.

Journal of experimental therapeutics & oncology·2002

Modulation of both endogenous folates and thymidine enhance the therapeutic efficacy of thymidylate synthase inhibitors.

Cancer research·2001

Pharmacokinetic and pharmacodynamic evaluation of the glycinamide ribonucleotide formyltransferase inhibitor AG2034.

Clinical cancer research : an official journal of the American Association for Cancer Research·2000

Accumulation of plasma reduced folates after folic acid administration.

Seminars in oncology·1999

Modulation of fluorouracil antitumor activity by folic acid in a murine model system.

Biochemical pharmacology·1999

Multiple mechanisms of resistance to polyglutamatable and lipophilic antifolates in mammalian cells: role of increased folylpolyglutamylation, expanded folate pools, and intralysosomal drug sequestration.

Molecular pharmacology·1999

HF-MMLLE USING NATURAL DEEP EUTECTIC SOLVENTS FOR DETERMINATION OF POTENTIAL LUNG CANCER BIOMARKERS IN URINE.

Analytical biochemistry·2026

Best practices for isothermal titration calorimetry of protein-DNA interactions.

Analytical biochemistry·2026

A CRISPR/Cas9 multiplex nucleic acid sensing platform based on rolling circle amplification-mediated monomeric substrate generation.

Analytical biochemistry·2026

Identification of MDV and REV Using RPA-CRISPR/Cas12a Combined with Fluorescence and Lateral Flow Assays.

Analytical biochemistry·2026

Selective fluorescence turn-on detection of methylglyoxal using N-substituted NBD derivatives.

Analytical biochemistry·2026

Simultaneous GC-MS determination of unsubstituted, hydroxy-, amino-, and hydroxy-amino short-chain fatty acids together with amino acids in biological and food samples following derivatization.

Analytical biochemistry·2026

Challenges and opportunities for the construction of the next IntCal and SHCal radiocarbon calibration curves.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026

"Green spectrophotometric determination of rupatadine, its toxic impurity, and methyl paraben: comparative AI-assisted univariate versus multivariate chemometrics".

Scientific reports·2026

Influence of sample size, content range, and sample removal on slope and bias correction in Fourier-transform mid-infrared spectroscopy for milk fatty acids.

Journal of dairy science·2026

Characterizing the Elemental Metabolome in Asparagus officinalis with Multielemental Speciation Analysis.

Journal of agricultural and food chemistry·2026

Analysis of ultra short-chain PFAS in urine: method development by LC-MS/MS and first human biomonitoring results from the German National Cohort (NAKO).

Environment international·2026

An accurate and sensitive method for identifying atmospheric alkylamines: selective enrichment by alkaline purging-acidic absorption coupled with ion chromatography-tandem mass spectrometry.

Journal of chromatography. A·2026

Related Experiment Videos

A centrifugal column assay for thymidylate synthetase using the active site titrant 5-fluoro-2'-deoxyuridylate

Related Concept Videos

Modulation of both endogenous folates and thymidine enhance the therapeutic efficacy of thymidylate synthase inhibitors.

Pharmacokinetic and pharmacodynamic evaluation of the glycinamide ribonucleotide formyltransferase inhibitor AG2034.

Accumulation of plasma reduced folates after folic acid administration.

Modulation of fluorouracil antitumor activity by folic acid in a murine model system.

Multiple mechanisms of resistance to polyglutamatable and lipophilic antifolates in mammalian cells: role of increased folylpolyglutamylation, expanded folate pools, and intralysosomal drug sequestration.

HF-MMLLE USING NATURAL DEEP EUTECTIC SOLVENTS FOR DETERMINATION OF POTENTIAL LUNG CANCER BIOMARKERS IN URINE.

Best practices for isothermal titration calorimetry of protein-DNA interactions.

A CRISPR/Cas9 multiplex nucleic acid sensing platform based on rolling circle amplification-mediated monomeric substrate generation.

Identification of MDV and REV Using RPA-CRISPR/Cas12a Combined with Fluorescence and Lateral Flow Assays.

Selective fluorescence turn-on detection of methylglyoxal using N-substituted NBD derivatives.

Simultaneous GC-MS determination of unsubstituted, hydroxy-, amino-, and hydroxy-amino short-chain fatty acids together with amino acids in biological and food samples following derivatization.

Challenges and opportunities for the construction of the next IntCal and SHCal radiocarbon calibration curves.

"Green spectrophotometric determination of rupatadine, its toxic impurity, and methyl paraben: comparative AI-assisted univariate versus multivariate chemometrics".

Influence of sample size, content range, and sample removal on slope and bias correction in Fourier-transform mid-infrared spectroscopy for milk fatty acids.

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Analysis of ultra short-chain PFAS in urine: method development by LC-MS/MS and first human biomonitoring results from the German National Cohort (NAKO).

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A centrifugal column assay for thymidylate synthetase using the active site titrant 5-fluoro-2'-deoxyuridylate

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