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Human basophil degranulation in dermatophagoides allergies: 93 cases
Annals of Allergy
|May 1, 1980
Summary
Human basophil degranulation (HBD) effectively diagnoses house dust allergies caused by Dermatophagoides. This reliable method shows high concordance with skin tests for Dermatophagoides pteronyssinus and Dermatophagoides farinae.
Area of Science:
- Immunology
- Allergology
Background:
- House dust mites, including Dermatophagoides pteronyssinus (DP) and Dermatophagoides farinae (DF), are common triggers for allergic reactions.
- Accurate diagnosis of house dust mite allergy is crucial for effective patient management.
Purpose of the Study:
- To evaluate the efficacy of the human basophil degranulation (HBD) test in diagnosing allergies to DP and DF.
- To assess the concordance between HBD results and traditional skin prick tests.
Main Methods:
- Human basophil degranulation (HBD) test performed on 93 subjects.
- Enrichment of circulating basophils, slide preparation, and microscopic counting of at least 100 basophils.
- Testing with allergen dilutions (100, 10, and 1 µg/ml) and defining positive HBD at >35% degranulation (p<0.001).
Main Results:
- High concordance between HBD and skin tests for DP (95%) and DF (86.6%).
- 27 out of 30 subjects with positive DP skin tests showed positive HBD.
- No significant correlation found between skin test diameter and HBD, potentially due to variable allergen potency.
Conclusions:
- Human basophil degranulation (HBD) is a simple, reliable, and effective method for diagnosing house dust mite allergies.
- HBD demonstrates strong diagnostic potential, complementing existing allergy testing methods.