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Related Experiment Videos

Citrate induced hypocalcaemia during cell separation

D K Watson, A F Penny, R W Marshall

    British Journal of Haematology
    |March 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

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    Adding calcium during cell separation with the Haemonetics Model 30 is crucial when using citrated plasma. Calcium prevents hypocalcemia and related symptoms, but is unnecessary with Plasma Protein Fraction replacement fluid.

    Area of Science:

    • Biomedical Engineering
    • Hematology
    • Clinical Chemistry

    Background:

    • Cell separation procedures, such as apheresis using the Haemonetics Model 30, often employ anticoagulants like citrate.
    • Citrate can chelate calcium, leading to citrate-induced hypocalcemia, a potentially serious complication.
    • Replacement fluids are used to maintain volume during cell separation.

    Purpose of the Study:

    • To evaluate the necessity of calcium supplementation during Haemonetics Model 30 cell separation.
    • To compare the effects of calcium addition with different replacement fluids: citrated plasma versus Plasma Protein Fraction.

    Main Methods:

    • Investigated two patient groups undergoing cell separation with the Haemonetics Model 30.
    • Assessed clinical symptoms and measured serum calcium levels.

    Related Experiment Videos

  • Compared outcomes when using citrated plasma with and without calcium addition.
  • Compared outcomes when using Plasma Protein Fraction as the replacement fluid.

    • Main Results:

    • In patients receiving citrated plasma, calcium addition effectively abolished clinical symptoms and reduced citrate-induced hypocalcemia.
    • When Plasma Protein Fraction was used as the replacement fluid, clinical symptoms and significant hypocalcemia did not occur, even without calcium addition.

    Conclusions:

    • Calcium supplementation is essential when citrated plasma is used as a replacement fluid during Haemonetics Model 30 cell separation to prevent hypocalcemia.
    • Calcium addition is not required when Plasma Protein Fraction is used, as it does not lead to significant hypocalcemia.