Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reversible dimerization of avian pancreatic polypeptide

P J Chang, M E Noelken, J R Kimmel

    Biochemistry
    |April 29, 1980
    PubMed
    Summary

    Avian pancreatic polypeptide (PP) dimerization is endothermic and entropically driven, suggesting hydrophobic interactions. This process is pH-dependent, involving ionizable groups and potentially salt linkages for avian PP.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Comparison of the accuracy of intraocular lens power calculation formulas based on the new swept-source optical coherence tomography biometry].

    [Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2021
    Same author

    Natural Language Processing Techniques for Extracting and Categorizing Finding Measurements in Narrative Radiology Reports.

    Applied clinical informatics·2015
    Same author

    Pilot study on the effect of composite UmayC in catheter-associated lower urinary tract infection.

    Urologia internationalis·2010
    Same author

    Evolution of organic aerosols in the atmosphere.

    Science (New York, N.Y.)·2009
    Same author

    The management of labour using continuous lumbar epidural analgesia with 0.2% ropivacaine in a parturient with traumatic brain injury.

    European journal of anaesthesiology·2005
    Same author

    Effect of sequence length, sequence frequency, and data acquisition rate on the performance of a Hadamard transform time-of-flight mass spectrometer.

    Journal of the American Society for Mass Spectrometry·2002

    Area of Science:

    • Biochemistry
    • Protein Chemistry

    Background:

    • Avian pancreatic polypeptide (PP) is a peptide hormone.
    • Dimerization is a key process influencing protein function.
    • Previous studies show pH-dependent dimerization in bovine and canine PP.

    Purpose of the Study:

    • To investigate the dimerization of avian pancreatic polypeptide (PP).
    • To determine the thermodynamic and kinetic parameters of avian PP dimerization.
    • To elucidate the role of pH and ionizable groups in avian PP dimerization.

    Main Methods:

    • Large-zone gel chromatography with integral boundary analysis.
    • Determination of the association constant as a function of temperature and pH.
    • Comparative analysis of amino acid sequences.

    Main Results:

    • Avian PP dimerization is endothermic and entropically driven, indicating hydrophobic interactions.
    • Dimerization is enhanced with increasing pH.
    • pH dependence suggests involvement of ionizable groups with pKa values of 4.5-5.5.
    • Salt linkages involving carboxylate and guanidinium groups are implicated, with histidine-34 potentially involved.

    Conclusions:

    • Avian PP dimerization is primarily driven by hydrophobic interactions and influenced by pH.
    • Ionizable groups, likely involving salt linkages, play a crucial role in the dimerization process.
    • Understanding these interactions provides insights into avian PP structure-function relationships.

    Related Experiment Videos