[Phosphorylation of creatine and the membrane potential of heart mitochondria]

The method of penetrating ions was used to measure the membrane potential and to study the creatine phosphokinase reaction in rat heart mitochondria. The concentration of adenine nucleotides in the intermembrane space at different levels of the membrane potential was estimated. The role of the diffusional layer adjacent to the inner membrane of heart mitochondria for coupling of the creatine phosphokinase reaction with oxidative phosphorylation is discussed.