The effect of NaCl on the upper temperature limit for growth of and enterotoxin synthesis by Staphylococcus aureus

The upper temperature limit for growth of an enterotoxin A producing strain (MF31) and of an enterotoxin B producing strain (S6) was about 44-45 degrees C in either a peptone-based medium or heart infusion broth. In the presence of NaCl, growth and production of enterotoxin, coagulase, and thermostable nuclease occurred up to 2 degrees C higher. The optimal concentration of NaCl for the temperature elevation effect was 1 M.