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[Analysis of 5'-deoxyribonucleotides by high-effective liquid chromatography]
Prikladnaia Biokhimiia I Mikrobiologiia
|March 1, 1980
Abstract:
The paper describes a method of analyzing 5'-deoxyribonucleotides by high-effective liquid chromatography on the strong-basic anion exchange resin Aminex A-27 in a nongradient phosphate borate buffer with ethanol addition. This method is useful for simultaneous separation of up to 6 UV-absorbing impurities--mononucleotides and nucleosides--with a sensitivity of 0.01 to 0.025 microgram.