Thymidine transport in hepatocytes. An assay for hepatotoxicity

Thymidine transport and phosphorylation were investigated in isolated rat hepatocytes and AS 30 D hepatoma cells. In contrast to hepatoma cells, hepatocytes exhibited a minimum of thymidine phosphorylation due to a 100-fold smaller thymidine kinase activity. In hepatocytes thymidine is transported by two transport systems: a specific concentrative "high affinity" system and an unspecific non-concentrative "low affinity" system. In hepatoma cells only the "low affinity" system could be detected. A single dose of 20 or 50 mg diethylnitrosamine/kg body weight induced in hepatocytes a remakable increas of thymidine kinase activity and a decrease of the transport by the "high affinity" system. Thymidine transport and phosphorylation by hepatocytes are considered to be sensitive markers for early recognition of toxin-induced liver regeneration.