[Malignant tumor induction in mice by methylcholanthrene covalently bound to bovine serum albumin]

The cancerogenic properties of 3-methylcholantrene covalently bound to protein through bis-diasotated benzidin were studied in mouse experiments. It has been shown that protein binding does not abolish the cancerogenic activity: subcutaneous administration of the conjugate gives rise to a rapid development of sarcomas at the injection sites. This makes difficult the immunization of the animals with the conjugated antigen 3-methylcholantrene-protein with a purpose of producing antibodies that would specifically bind the cancerogenic substance.