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Updated: Aug 4, 2026

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
Published on: August 22, 2019
Time dependent changes induced by external perturbations on a low molecular weight RNA
Abstract:
The behaviour of a soluble RNA (MW 19,000) extracted from calf thymus was studied in a partially aqueous solvent. A mixture of dioxane and an aqueous solution of 50 mM NaCl (10:1) was used to dissolve the RNA and the slow optical changes of the solution depending on time and RNA concentration were followed. The relaxation of the RNA was measured after a T-jump in the dioxane-NaCl solvent with an addition of 7 mM methyl red at 470 nm. The relaxation time was measured after experiments carried out with different RNA concentration and the rate process appears to be independent of the RNA concentration.
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