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Related Experiment Videos

Ultrastructural changes at gap junctions between lesioned crayfish axons

G D Bittner, M L Ballinger

    Cell and Tissue Research
    |January 1, 1980
    PubMed
    Summary

    Severed single lateral giant axon (SLGA) distal segments in crayfish survive long-term when adjacent to intact SLGAs, suggesting trophic support. This support likely involves vesicle-mediated transport, not gap junctions, which disappear after injury.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Crustacean Biology

    Background:

    • Single lateral giant axons (SLGAs) in crayfish are crucial for escape responses.
    • Axonal injury can lead to degeneration, but some segments may survive under specific conditions.

    Purpose of the Study:

    • To investigate the long-term survival and ultrastructural changes of severed SLGA distal segments in crayfish.
    • To explore the potential mechanisms of trophic support maintaining severed SLGA viability.

    Main Methods:

    • Lesioning of SLGAs in crayfish.
    • Ultrastructural analysis using electron microscopy at various time points post-lesioning (1 day to 10 months).
    • Comparison of severed SLGA segments with adjacent intact SLGAs.

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    Main Results:

    • Severed SLGA distal segments can survive for at least 10 months if adjacent to an intact SLGA.
    • Ultrastructural changes (mitochondria, neurotubules) are observed in severed segments within 14 days.
    • Gap junctions between intact and severed SLGAs largely disappear post-lesioning.
    • Surviving severed SLGA segments show normal appearance but lack gap junction-associated structures.
    • 50-60 nm vesicles are consistently present at the septum between intact and severed SLGAs.

    Conclusions:

    • Adjacent intact SLGAs provide trophic support to severed SLGA distal segments.
    • The primary mechanism of trophic support is likely not via gap junction diffusion due to their disappearance.
    • Exocytotic-pinocytotic activity of 50-60 nm vesicles at the septum is a probable route for trophic maintenance.