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Related Experiment Videos

Chemotactic peptide receptor modulation in polymorphonuclear leukocytes

S J Sullivan, S H Zigmond

    The Journal of Cell Biology
    |June 1, 1980
    PubMed
    Summary
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    Chemotactic peptide N-formylnorleucylleucylphenylalanine (FNLLP) binding to rabbit polymorphonuclear leukocytes (PMNs) causes receptor down-regulation and increased peptide uptake. This process is concentration and temperature-dependent, suggesting receptor-mediated pinocytosis.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Polymorphonuclear leukocytes (PMNs) play a crucial role in immune responses.
    • Chemotactic peptides, like N-formylnorleucylleucylphenylalanine (FNLLP), are key signaling molecules in inflammation.
    • Understanding receptor dynamics is vital for modulating immune cell function.

    Purpose of the Study:

    • To investigate the effects of FNLLP binding on its receptor number on rabbit PMNs.
    • To characterize the phenomenon of receptor down-regulation induced by FNLLP.
    • To explore the relationship between receptor modulation and peptide uptake.

    Main Methods:

    • Incubation of rabbit PMNs with varying concentrations of FNLLP.
    • Measurement of subsequent peptide binding capacity to assess receptor availability.

    Related Experiment Videos

  • Quantification of irreversibly cell-associated peptide.
  • Assessment of [3H]sucrose uptake to measure pinocytosis rates.

    • Main Results:

    • FNLLP binding leads to a concentration-dependent decrease in available peptide receptors (down-regulation).
    • Receptor down-regulation occurs rapidly, reaching equilibrium within 15 minutes, and is temperature-dependent.
    • Irreversible peptide association with cells increases with temperature and can exceed initial receptor numbers.
    • FNLLP stimulates a fourfold increase in pinocytosis, indicating receptor-mediated uptake.

    Conclusions:

    • FNLLP binding induces significant down-regulation of its own receptor on PMNs.
    • The observed peptide uptake is receptor-mediated and may involve pinocytosis.
    • These findings reveal novel mechanisms of immune cell response to chemotactic signals.