Analysis of a marine fish cell line from a male sheepshead

Chromosomes from consecutive culture passages of a developing cell line from fin fibroblasts of a male sheepshead (Archosargus probatocephalus) were analyzed. It was demonstrated that the modal chromosome number is 48. The chromosome types found in these cells included 8 submetacentrics, 8-12 acrocentrics, and 28-32 telocentrics. Evidence suggests that the primary type of chromosomal alteration that occurred in cultured SHF-1 cells is aneuploidy; a large metacentric chromosome of undetermined origin was observed.