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Related Experiment Videos

Stability of hemoglobin solution during extended storage

F DeVenuto

    The Journal of Laboratory and Clinical Medicine
    |December 1, 1978
    PubMed
    Summary
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    Long-term storage of hemoglobin solutions is crucial for blood substitutes. Freezing hemoglobin at -20°C ensures stability for two years, while refrigeration at 4°C maintains it for one year.

    Area of Science:

    • Biochemistry
    • Biotechnology
    • Hematology

    Background:

    • Hemoglobin solutions are investigated as potential blood substitutes.
    • Ensuring long-term stability is essential for stockpiling and immediate availability during massive transfusions.

    Purpose of the Study:

    • To evaluate the stability of crystallized hemoglobin solutions under various storage conditions.
    • To determine the optimal storage temperature for maintaining hemoglobin's functional and structural integrity over time.

    Main Methods:

    • Crystallized hemoglobin solutions were prepared and stored in sterile blood bags at -20°C, 4°C, and 25°C.
    • Key parameters including methemoglobin content, p50, n values, osmolality, oxygen capacity, Na, K, and pH were periodically assayed.

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    Main Results:

    • Hemoglobin solutions stored at -20°C showed no significant alterations in assayed parameters after two years.
    • Solutions stored at 4°C remained stable for 12 months, but showed deterioration (increased methemoglobin, decreased P50) after 18 months.
    • Storage at 25°C was unsuccessful in maintaining hemoglobin's structure and function.

    Conclusions:

    • Cryopreservation at -20°C is a viable method for long-term storage of hemoglobin solutions as blood substitutes.
    • Refrigeration at 4°C offers limited stability, and room temperature storage is not feasible.
    • Hemoglobin derived from fresh or outdated blood exhibits similar characteristics for this application.