DNA and histone synthesis in mouse cells which exhibit temperature-sensitive DNA synthesis

It is demonstrated that temperature inactivation of histone synthesis is coupled to inhibition of DNA replication in ts AlS9 and ts Cl mouse L-cells, which are temperature-sensitive (ts) in an S-phase function. In contrast, uncoupling of histone and DNA synthesis occurs in BalB/C-3T3 ts 2 cells which are ts in a function of the pre-DNA-synthetic phase. Termination of histone synthesis in ts AlS9 and ts Cl cells is 16--18 h after onset of temperature inactivation of DNA replication and appears to be associated with general cessation of chromatin replication triggered by the earlier event. Synthesis of histone and other chromosomal proteins proceeds in ts 2 cells under conditions in which DNA synthesis undergoes temperature inactivation. It is suggested that the terminal phenotype of coupled temperature inactivation of DNA and histone synthesis may be diagnostic of cells ts in an S-phase function and may therefore be a useful secondary screen in designation of cell cycle mutants.