Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium-binding proteins and renal lithiasis

M I Resnick, C W Gammon, M B Sorrell

    Surgery
    |August 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The medical bookshelf.

    Postgraduate medicine·2016
    Same author

    Single photon emission computerized tomography with capromab pendetide plus computerized tomography image set co-registration independently predicts biochemical failure.

    The Journal of urology·2008
    Same author

    Rapid onset of action with alfuzosin 10 mg once daily in men with benign prostatic hyperplasia: a randomized, placebo-controlled trial.

    Prostate cancer and prostatic diseases·2007
    Same author

    Experimental rodent models of prostatitis: limitations and potential.

    Prostate cancer and prostatic diseases·2007
    Same author

    Fibrous inflammatory pseudotumor of the bladder.

    Reviews in urology·2006
    Same author

    Therapeutic strategies for localized prostate cancer I: surgery, ultrasound, adjuvant and neoadjuvant therapy.

    Reviews in urology·2006

    Active calcium oxalate stone formers exhibit higher urinary calcium-binding protein activity than inactive stone formers or controls. This suggests a link between calcium binding and kidney stone activity.

    Area of Science:

    • Nephrology
    • Biochemistry
    • Urology

    Background:

    • Kidney stones, particularly calcium oxalate stones, are a significant health concern.
    • Understanding the biochemical factors contributing to stone formation is crucial for developing effective prevention strategies.

    Purpose of the Study:

    • To investigate urinary calcium-binding activity in patients with different types of kidney stones and in healthy controls.
    • To determine if calcium-binding activity correlates with stone formation activity, specifically in calcium oxalate stone formers.

    Main Methods:

    • Collected 24-hour urine samples from active and inactive calcium oxalate stone formers, active uric acid and cystine stone formers, and nonstone-forming controls.
    • Ultrafiltered urine to concentrate macromolecules (<50,000 daltons) and separated fractions using gel filtration chromatography.

    Related Experiment Videos

  • Assessed calcium-binding activity in each fraction using the chelex resin binding assay.

    • Main Results:

    • Total urinary calcium-binding protein activity was significantly elevated in active calcium oxalate stone formers compared to inactive stone formers (P < 0.025) and controls (P < 0.05).
    • Comparisons for uric acid and cystine stone formers were limited due to small sample sizes.
    • Urinary calcium binding appears to correlate with kidney stone activity.

    Conclusions:

    • Urinary calcium-binding activity is significantly higher in active calcium oxalate stone formers.
    • This elevated activity may be specific to calcium stone formers and warrants further investigation into its role in stone pathogenesis.
    • Further research is needed to elucidate the precise mechanisms and clinical implications of urinary calcium binding in kidney stone formation.