Plasma free amino acid level in subjects during maximal workload

Ten healthy young males were subjected to one-time physical exercise on cycle ergometer up to exhaustion. For the investigations blood samples were obtained twice from a vein before and after exercise. In the venous blood the levels of lactic acid and pyruvic acid were determined by enzymatic methods. In heparized blood plasma the composition of free amino acids was determined using an autoanalyzer Beckmann-Multichrom. The following amino acids were determined: lys, his, arg, asp, tre, ser, glu, pro, gli, ala, cys, val, met, leu, ileu, tyr, phen. The obtained results were subjected to statistical analysis by the paired test of Student. The observed rise in the concentration after exercise concerned alanine, glutamate and phenylalanine. The concentrations of the remaining amino acids were not statistically significantly changed after the exercise.