Base composition of transfer RNAs from vegetative and developing cells of Dictyostelium discoideum

In a previous comparison of tRNAs from vegetative and developing cells of Dictyostelium discoideum, displacements of chromatographic peaks were observed for a number of aminoacyl-tRNAs (Palatnik, C.M., Katz, E.R. and Brenner, M. (1977) J. Biol. Chem. 252, 694-703). Since the development peak eluted ahead of the vegetative peak in all but one case, and since levels of amino acid acceptance and tRNA chromatographic profiles were otherwise quite similar, it was suggested that a common change or a very small number of changes in base modification were occurring during development. We have threfore compared tRNAs from both stages with respect particularly to their minor base composition. Of the minor bases which we have analyzed no reproducible differences between the two stages have been detected. Moreover, we have been unable to confirm a recent report (Dingermann, T., Schmidt, W. and Kersten, H. (1977) FEBS Lett. 80, 205-208) that a significant change occurs in the ribothymidine content of tRNA during Dictyostelium discoideum development.