Preliminary results of J-resolved, two-dimensional 1H-NMR studies on glycosphingolipids

Homonuclear J-resolved two-dimensional spectroscopy has been applied to the simplest glycosphingolipid, glycosylceramide. In comparison with the conventional one-dimensional spectrum, the possible advantages and shortcomings of the two-dimensional method as applied to blood-group-active glycosphingolipids consisting of several saccharide units, have been pr esented. As an example, preliminary results on a more complex pentaosylceramide (IV3Gal-alpha-neolactotetraosylceramide) are discussed.