Beta-D-mannanases in germinating lucerne (alfalfa) seeds

Area of Science:

• Biochemistry
• Enzymology

Background:

• Beta-D-mannanases are enzymes involved in plant cell wall metabolism.
• Understanding enzyme formation during germination is crucial for plant physiology research.

Purpose of the Study:

• To investigate the formation and characteristics of beta-D-mannanase fractions during lucerne germination.
• To determine if beta-D-mannanase fractions are formed in vivo or post-extraction.

Main Methods:

• Enzyme extraction from germinating lucerne.
• Fractionation using ion-exchange chromatography (DEAE-cellulose).
• Molecular weight separation via gel chromatography.

Main Results:

• Four beta-D-mannanase fractions were obtained when extracts were stored before chromatography.
• A high-molecular-weight fraction was detected only when extracts were stored, indicating post-extraction formation.
• Bound and unbound fractions increased and then decreased during germination, with stable ratios.

Conclusions:

• Three beta-D-mannanase fractions are likely formed in vivo prior to plant tissue maceration.
• One beta-D-mannanase fraction appears to be formed after the extraction process.