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Induction of intranuclear microtubules in chick embryo fibroblasts by frog virus 3
Abstract:
Intranuclear microtubules appear in chick embryo fibroblasts upon infection with Frog Virus 3 (FV 3). Both the diameter and the annular shape of the microtubule profiles, established from electron microscopic observations using a goniometer, suggest that they are identical to naturally occurring cytoplasmic microtubules. Furthermore, the use of vinblastine allowed demonstration of the tubulin composition of the intranuclear microtubules.
Insights
Intranuclear microtubules were discovered in chick embryo fibroblasts after Frog Virus 3 (FV 3) infection. These viral-induced intranuclear microtubules share characteristics with cytoplasmic microtubules and are composed of tubulin.
Area of Science:
- Cell Biology
- Virology
- Microscopy
Background:
- Microtubules are essential cytoskeletal components involved in various cellular processes.
- Viral infections can significantly alter host cell structure and function.
Purpose of the Study:
- To investigate the presence and characteristics of microtubules within the nucleus of infected cells.
- To determine the composition of these intranuclear microtubules.
Main Methods:
- Electron microscopy was employed to observe cellular structures.
- Goniometer-equipped microscopy provided detailed analysis of microtubule profiles.
- Treatment with vinblastine was used to assess microtubule composition.
Main Results:
- Intranuclear microtubules were identified in chick embryo fibroblasts following Frog Virus 3 infection.
- Microscopic analysis revealed that these intranuclear microtubules possess similar diameters and annular shapes to cytoplasmic microtubules.
- Vinblastine treatment confirmed the presence of tubulin, indicating a shared composition with cytoplasmic microtubules.
Conclusions:
- Frog Virus 3 infection induces the formation of intranuclear microtubules in host cells.
- These viral-induced intranuclear microtubules are structurally and compositionally similar to endogenous cytoplasmic microtubules.