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Updated: Aug 7, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Published on: October 9, 2009
Bacteriophage T7 DNA replication in vitro. Electron micrographic analysis of T7 DNA synthesized with purified
Abstract:
Extensive replication of duplex T7 DNA is catalyzed in reactions contining T7 DNA polymerase, T7 gene 4 protein, and T7 RNA polymerase. When the product of this reaction is analyzed in the electron microscope, many eye form and Y form replication intermediates are observed. Replication in vitro is not initiated at a single region of the T7 genome. However, we tentatively conclude that initiation does occur preferentially at a few specific sites along the DNA, and that these sites may be near promoters at which the T7 RNA polymerase initiates transcription.
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