Secretion of newly synthesized proteins by isolated liver cells

Suspensions of isolated liver cells were incubated with [3H]leucine as a precursor. The appearance of radioactive albumin and alpha 1-acid glycoprotein in the incubation medium was determined. After about 20 min both albumin and alpha 1-acid glycoprotein were secreted in a linear way. When fucose and galactose were used as radioactive precursors, time lags of about 10 min could be observed.