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Related Experiment Videos

LH bioactivity increases more than immunoreactivity during puberty

A W Lucky, B H Rich, R L Rosenfield

    The Journal of Pediatrics
    |August 1, 1980
    PubMed
    Summary

    Bioactive luteinizing hormone (B-LH) increases significantly more than immunoreactive LH (I-LH) during puberty in both boys and girls. This suggests B-LH is a better indicator of adolescent sexual maturation.

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    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Pediatric Endocrinology

    Background:

    • Standard measurement of luteinizing hormone (LH) via radioimmunoassay (immunoreactive LH or I-LH) may underestimate the hormone's activity during puberty.
    • The significant physical changes during adolescence suggest a more substantial hormonal shift than I-LH levels alone indicate.

    Purpose of the Study:

    • To compare the changes in bioactive LH (B-LH) and I-LH during normal pubertal development in boys and girls.
    • To determine which LH measurement, B-LH or I-LH, better reflects pubertal maturation.

    Main Methods:

    • Plasma samples from 60 boys and 45 girls were analyzed for both B-LH and I-LH throughout puberty.
    • Bioactive LH was quantified using an in vitro bioassay measuring rat interstitial cell testosterone production.

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  • Immunoreactive LH was measured by radioimmunoassay using the standard LER 907.

    • Main Results:

    • Bioactive LH levels increased substantially more than I-LH levels in both sexes compared to chronological age, bone age, and pubertal stage.
    • In boys, B-LH rose 8.2-fold and I-LH rose 3.0-fold from prepuberty to late puberty.
    • In girls, B-LH increased 23.1-fold and I-LH increased 4.9-fold during the same period.
    • B-LH showed less overlap between pubertal stages than I-LH, indicating greater discriminatory power.

    Conclusions:

    • Bioactive LH exhibits a greater increase than immunoreactive LH during normal puberty.
    • B-LH is a more sensitive and discriminating marker of pubertal maturation than I-LH.
    • These findings suggest a potential qualitative change in gonadotropin secretion during adolescence.