[Method of long-term electrophoresis in gradient polyacrylamide gel with a linear change of acrylamide concentration]

The method of long-time protein electrophoresis in a gradient polyacrylamide gel with a linear change of acrylamide concentration along the gel column was used to determine the molecular mass of protein molecules and their geometric sizes. In our investigation great attention was paid to the determination of the molecular form by the peak width in densitograms of protein fractions. Besides experimental data a possible physical interpretation of the essense of empiric equation is suggested.