[Structural features of isolated and perfused neurons of Helix pomatia mollusks]

Ultrastructural features of the intact normal neurons of Helix pomatia L., were studied and compared with those of isolated and perfused neurons. The perfusion of the isolated neurons had no definite influence on the intracellular organelia membranes--endoplasmic reticulum, Golgi complex, the neurosecretary granules, pigment bodies. Some changes were manifested in the vacuolization of the structures, increase in the number of cytosomes and lysosomes, modification of the size and shape of the neurosecretory granules. No visible ultrastructural changes of the superficial membrane, submembrane parts of cytoplasm were observed after intracellular perfusion. The obtained data confirm the use of perfused neurons as a model for the investigation of electrophysiological properties of the somatic membrane.