Renal prostaglandin E2 and D2 alpha secretion and excretion in exercising conscious dogs

Renal prostaglandin E2 (PGE2) and F2 alpha (PGF2 alpha) secretion and excretion rates were determined in dogs at rest and during various levels of treadmill exercise. Four animals were chronically instrumented to measure mean arterial pressure (MAP), heart rate (HR), left renal blood flow (RBF), and to sample renal venous (RV) blood. In four additional animals, the bladder was cannulated to enable urine collection prior to and after exercise. When compared to resting values, RV PGE2 concentrations increased by 99, 119, and 50 percent during light, moderate and heavy exercise workloads, respectively. RBF was constant during exercise and consequently renal PGE2 secretion increased as a function of RV PGE2 concentration. Similar changes were observed in PGF2 alpha in 2 dogs. When compared to resting values, excretion rates of PGE2 respectively, due to an increase in urine PGE2 and PGF2 alpha concentrations. The data demonstrate that renal PG synthesis, as evaluated by PG secretion and excretion, is increased during exercise in the dog.