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Decrease in heart contractility produced by ouabain in fibers exposed to theophylline

W C De Mello, M Gonzalez-Castillo

    Research Communications in Chemical Pathology and Pharmacology
    |June 1, 1980
    PubMed
    Summary
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    Theophylline significantly reduced ouabain's positive inotropic effects on cardiac muscle. Ouabain

    Area of Science:

    • Pharmacology
    • Cardiovascular Physiology

    Background:

    • Ouabain is a cardiac glycoside known for its positive inotropic effects.
    • Theophylline is a methylxanthine drug with complex cardiovascular actions.

    Purpose of the Study:

    • To investigate the influence of theophylline on the inotropic action of ouabain in mammalian cardiac muscle.

    Main Methods:

    • Experiments were conducted on isolated strips of mammalian cardiac muscle.
    • Preparations were exposed to theophylline (4 mM) followed by ouabain (2 X 10(-6) M).
    • Measurements included peak tension, rate of tension development (dF/dt)max, and rate of twitch relaxation (dR/dt).

    Main Results:

    • Theophylline pretreatment significantly attenuated ouabain's effects.

    Related Experiment Videos

  • Peak tension was reduced by 56%.
  • The rate of tension development (dF/dt)max decreased by 44%, and the rate of twitch relaxation (dR/dt) by 65%.

    • Conclusions:

    • Theophylline exhibits a negative inotropic effect when combined with ouabain.
    • This suggests a complex interaction between theophylline and cardiac glycosides.
    • Further research is needed to elucidate the precise mechanisms underlying this interaction.