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Infectious drug resistance during an outbreak of samonellosis
Abstract:
The sudden acquisition of aminoglycoside resistance among Salmonella group C1 isolates causing summer diarrhoea raised the possibility oif plasmid-mediated reistance. The demonstration of circular DNA species in the resistant, but not in the sensitive salmonellae and the transfer by conjugation of antibiotic resistance to a sensitive strain of Escherichic coli, was consistent with plasmid-mediated resistance.
Insights
Aminoglycoside resistance in Salmonella group C1 emerged rapidly, suggesting plasmid-mediated transfer. Researchers confirmed this by identifying plasmids in resistant bacteria and demonstrating resistance transfer to E. coli.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Salmonella group C1 isolates causing summer diarrhea exhibited sudden aminoglycoside resistance.
- This rapid emergence suggested a potential mechanism of plasmid-mediated resistance.
Purpose of the Study:
- To investigate the mechanism behind the sudden acquisition of aminoglycoside resistance in Salmonella group C1.
- To determine if the resistance was mediated by plasmids.
Main Methods:
- Detection of circular DNA species (plasmids) in resistant and sensitive Salmonella isolates.
- Conjugation experiments to transfer antibiotic resistance from resistant Salmonella to a sensitive strain of Escherichia coli.
Main Results:
- Circular DNA species were identified in resistant Salmonella isolates, but not in sensitive ones.
- The transfer of antibiotic resistance to a sensitive Escherichia coli strain was successfully demonstrated via conjugation.
Conclusions:
- The findings are consistent with plasmid-mediated aminoglycoside resistance in Salmonella group C1.
- Plasmids play a significant role in the dissemination of antibiotic resistance within bacterial populations.