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The action of anti-A,AB isoantibodies on group A, AB-positive human lymphocytes
Human lymphocytes of group A and group AB individuals were exposed to anti-A,B antisera both in growth medium and in fresh autologous plasma. Agglutination developed slowly but was present in all positive specimens by four hours and was negative in the group O controls even after 24 hours. There was no loss of viability even in the presence of the fresh plasma. The lymphocytes in the agglutinates appeared larger and more irregular suggesting stimulation of the lymphocytes but assessment by 3H thymidine uptake showed no evidence of a mitogenic response.
Human lymphocytes of group A and group AB individuals were exposed to anti-A,B antisera both in growth medium and in fresh autologous plasma. Agglutination developed slowly but was present in all positive specimens by four hours and was negative in the group O controls even after 24 hours. There was no loss of viability even in the presence of the fresh plasma. The lymphocytes in the agglutinates appeared larger and more irregular suggesting stimulation of the lymphocytes but assessment by 3H thymidine uptake showed no evidence of a mitogenic response.