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The ultrastructure of collagenous fibers in bone marrow long-term cultures
Abstract:
The bone marrow of resected bones was studied by electron microscopy before and during cultivation on the 9th, 13th and 15th day of explantation. The absence of collagenous fibers in the inoculum and its appearance in individual sites in 9-day cultures simultaneously with growth of fibroblasts, in numerous sites in 13-day cultures and in almost all sites in 15-day cultures point to the appearance and growth of collagenous fibers after a certain cultivation time. This is also confirmed by the appearance, in the latest cultures, of collagenous fibers with transverse striation, which indicates that they mature during cultivation. The results obtained may serve as evidence of growth of collagen-producing cells (true fibroblasts) in the above-mentioned cultures.
Insights
Collagenous fibers and mature fibroblasts appear in bone marrow cultures over time. This indicates that bone marrow contains cells capable of producing collagen during cultivation.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Tissue Engineering
Background:
- Bone marrow contains mesenchymal stem cells.
- Fibroblasts are crucial for collagen production in bone tissue.
- Understanding cell behavior in vitro is vital for regenerative medicine.
Purpose of the Study:
- To investigate the in vitro growth and maturation of collagenous fibers from bone marrow explants.
- To identify the presence and activity of collagen-producing cells in cultivated bone marrow.
Main Methods:
- Electron microscopy was used to examine bone marrow samples.
- Samples were cultured for 9, 13, and 15 days post-explantation.
- Cultures were analyzed for the presence and morphology of collagenous fibers and fibroblasts.
Main Results:
- Collagenous fibers were absent in initial explants but appeared by day 9 of cultivation.
- The quantity and distribution of collagenous fibers increased significantly by days 13 and 15.
- Mature collagenous fibers with transverse striations were observed in later cultures, indicating fiber development.
Conclusions:
- Bone marrow explant cultures support the growth and maturation of collagenous fibers.
- The study provides evidence for the presence of functional, collagen-producing fibroblasts within bone marrow cultures.