Analysis of protein levels and synthesis after learning in the split-brain pigeon

A series of experiments were performed to study the effects of learning upon proteins in the split-brain pigeon. With this preparation, one side of the brain was trained in a color discrimination task while the other side served as a naive control. Proteins from both sides of the brain were separated by one- and two-dimensional polyacrylamide gel electrophoresis and analyzed to measure protein levels or protein synthesis. No differences were found between trained and naive telencephalic hemispheres or between trained and naive optic lobes in the steady-state protein levels or in protein synthesis.