Serum protein profiles by "high-performance" liquid chromatography with detection at multiple wavelengths
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Updated: Aug 19, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
Abstract:
We have separated serum proteins in only 15 min by high-performance liquid chromatography. The identification of several peaks in the chromatographic profile was greatly aided by the use of multiple-wavelength detection. We have found a good correlation between retention times and electrophoretic mobilities. Serum samples with increased gamma- or beta-globulins, as determined by electrophoresis, resulted in chromatographic profiles with strongly increased peaks in the appropriate regions. This chromatographic method revealed both relative and absolute differences in individual protein concentrations among several serum samples.
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