Radioimmunoassay for melatonin

A radioimmunoassay for melatonin has been developed and used to measure the level of melatonin of male and post-menopausal female patinets coming to operation for benign and malignant conditions. The amount of melatonin in the serum of the females was considerably lower than that in males. No difference could be found between patients suffering from benign and malignant conditions. A patient with a non-parenchymatous pineal tumour had considerably lower levels in the serum at three months after surgery and radiotherapy. A further month later melatonin could not be found in samples of serum taken over a 24-hour period.