Efficiency and fidelity of cell-free protein synthesis by transfer RNA from aged mice

Transfer RNAs (tRNAs) from heart, kidney, liver, and spleen of mature (10-12 months old) and aged (29 months old) C57BL/6 mice were tested for their ability to translate encephalomyocarditis viral RNA in a tRNA-dependent cell-free system derived from mouse ascites tumor cells. The rates of in vitro protein synthesis were compared as a function of tRNA concentration, and the fidelity of translation was examined by sodium dodecyl sulfate gel electrophoresis and isoelectric focusing of the viral polypeptides synthesized in vitro. No significant age-related differences in either the efficiency or fidelity of synthesis were discovered, indicating that alterations in tRNAs are probably not involved in the cellular aging of these tissues.